Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication

Eiji Abe, Kazuhiko Kuwahara, Mikoto Yoshida, Mikio Suzuki, Hidenori Terasaki, Yoshinobu Matsuo, Ei Ichi Takahashi, Nobuo Sakaguchi

研究成果: Article査読

39 被引用数 (Scopus)

抄録

A 210 kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7 kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. (C) 2000 Elsevier Science B.V. All rights reserved.

本文言語English
ページ(範囲)219-227
ページ数9
ジャーナルGene
255
2
DOI
出版ステータスPublished - 19-09-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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