Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein

Masaya Ohta, Jiharu Hamako, Satoru Yamamoto, Hajime Hatta, Mujo Kim, Takehiko Yamamoto, Satoru Oka, Tsuguo Mizuochi, Fumito Matsuura

研究成果: Article査読

76 被引用数 (Scopus)

抄録

Asparagine-linked oligosaccharides present on hen egg-yolk immunoglobulin, termed IgY, were liberated from the protein by hydrazinolysis. After N-acetylation, the oligosaccharides were labelled with a UV-absorbing compound, p-aminobenzoic acid ethyl ester (ABEE). The ABEE-derivatized oligosaccharides were fractionated by anion exchange, normal phase and reversed phase HPLC, and their structures were determined by a combination of sugar composition analysis, methylation analysis, negative ion FAB-MS, 500 MHz1H-NMR and sequential exoglycosidase digestions. IgY contained monoglucosylated oligomannose type oligosaccharides with structures of Glcα1-3Man7-9-GlcNAc-GlcNAc, oligomannose type oligosaccharides with the size range of Man5-9GlcNAc-GlcNAc, and biantennary complex type oligosaccharides with core region structure of Manα1-6(±GlcNAcβ1-4)(Manα1-3)Manβ1-4GlcNAcβ1-4(±Fucα1-6)GlcNAc. The glucosylated oligosaccharides, Glc1Man8GlcNAc2 and Glc1Man7GlcNAc2, have not previously been reported in mature glycoproteins from any source.

本文言語English
ページ(範囲)400-413
ページ数14
ジャーナルGlycoconjugate Journal
8
5
DOI
出版ステータスPublished - 10-1991

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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