抄録
During cardiopulmonary bypass (CPB), neutrophils (PMNs) may be stimulated by shear stress which could contribute to the pulmonary injury that occurs after CPB. To elucidate whether mechanically stimulated PMNs increase pulmonary vascular permeability, measured as the pulmonary filtration coefficient (K) and pulmonary vascular resistance, and to elucidate whether superoxide anion mediates this increase, we assessed the effects of stimulated and unstimulated PMNs, and of superoxide dismutase (SOD) on K and resistance in isolated perfused lungs from Sprague-Dawley rats. PMNs were stimulated by gentle agitation in a glass vial for 10s. Lungs perfused with the stimulated PMNs, being the stimulated group (n = 6), elicited a 5-fold increase in the filtration coefficient compared with lungs perfused with unstimulated cells, being the unstimulated group (n = 6). This increase in filtration was completely blocked by the preincubation of stimulated PMNs with CD18 monoclonal anti-body, being the Ab group (n = 6), and also by superoxide dismutase, being the SOD group (n = 6). Pulmonary vascular resistance was not increased by stimulated PMNs, and the accumulation of stimulated PMNs was not blocked by SOD. These findings suggest that stimulated PMNs increase K and that superoxide anion may injure the pulmonary vascular endothelial cells.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 755-761 |
| ページ数 | 7 |
| ジャーナル | Surgery Today |
| 巻 | 29 |
| 発行部数 | 8 |
| DOI | |
| 出版物ステータス | Published - 03-09-1999 |
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All Science Journal Classification (ASJC) codes
- Surgery
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Superoxide anion mediates pulmonary vascular permeability caused by neutrophils in cardiopulmonary bypass. / Tanita, Tatsuo; Song, Chun; Kubo, Hiroshi; Hoshikawa, Yasushi; Chida, Masayuki; Suzuki, Satoshi; Ono, Sadafumi; Fujimura, Shigefumi.
：: Surgery Today, 巻 29, 番号 8, 03.09.1999, p. 755-761.研究成果: Article
TY - JOUR
T1 - Superoxide anion mediates pulmonary vascular permeability caused by neutrophils in cardiopulmonary bypass
AU - Tanita, Tatsuo
AU - Song, Chun
AU - Kubo, Hiroshi
AU - Hoshikawa, Yasushi
AU - Chida, Masayuki
AU - Suzuki, Satoshi
AU - Ono, Sadafumi
AU - Fujimura, Shigefumi
PY - 1999/9/3
Y1 - 1999/9/3
N2 - During cardiopulmonary bypass (CPB), neutrophils (PMNs) may be stimulated by shear stress which could contribute to the pulmonary injury that occurs after CPB. To elucidate whether mechanically stimulated PMNs increase pulmonary vascular permeability, measured as the pulmonary filtration coefficient (K) and pulmonary vascular resistance, and to elucidate whether superoxide anion mediates this increase, we assessed the effects of stimulated and unstimulated PMNs, and of superoxide dismutase (SOD) on K and resistance in isolated perfused lungs from Sprague-Dawley rats. PMNs were stimulated by gentle agitation in a glass vial for 10s. Lungs perfused with the stimulated PMNs, being the stimulated group (n = 6), elicited a 5-fold increase in the filtration coefficient compared with lungs perfused with unstimulated cells, being the unstimulated group (n = 6). This increase in filtration was completely blocked by the preincubation of stimulated PMNs with CD18 monoclonal anti-body, being the Ab group (n = 6), and also by superoxide dismutase, being the SOD group (n = 6). Pulmonary vascular resistance was not increased by stimulated PMNs, and the accumulation of stimulated PMNs was not blocked by SOD. These findings suggest that stimulated PMNs increase K and that superoxide anion may injure the pulmonary vascular endothelial cells.
AB - During cardiopulmonary bypass (CPB), neutrophils (PMNs) may be stimulated by shear stress which could contribute to the pulmonary injury that occurs after CPB. To elucidate whether mechanically stimulated PMNs increase pulmonary vascular permeability, measured as the pulmonary filtration coefficient (K) and pulmonary vascular resistance, and to elucidate whether superoxide anion mediates this increase, we assessed the effects of stimulated and unstimulated PMNs, and of superoxide dismutase (SOD) on K and resistance in isolated perfused lungs from Sprague-Dawley rats. PMNs were stimulated by gentle agitation in a glass vial for 10s. Lungs perfused with the stimulated PMNs, being the stimulated group (n = 6), elicited a 5-fold increase in the filtration coefficient compared with lungs perfused with unstimulated cells, being the unstimulated group (n = 6). This increase in filtration was completely blocked by the preincubation of stimulated PMNs with CD18 monoclonal anti-body, being the Ab group (n = 6), and also by superoxide dismutase, being the SOD group (n = 6). Pulmonary vascular resistance was not increased by stimulated PMNs, and the accumulation of stimulated PMNs was not blocked by SOD. These findings suggest that stimulated PMNs increase K and that superoxide anion may injure the pulmonary vascular endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0032782904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032782904&partnerID=8YFLogxK
U2 - 10.1007/s005950050504
DO - 10.1007/s005950050504
M3 - Article
C2 - 10483751
AN - SCOPUS:0032782904
VL - 29
SP - 755
EP - 761
JO - Surgery Today
JF - Surgery Today
SN - 0941-1291
IS - 8
ER -