Superoxide possibly produced in endothelial cells mediates the neutrophil-induced lung injury

Tatsuo Tanita, Chun Song, Hiroshi Kubo, Yasushi Hoshikawa, Shinsaku Ueda, Shigefumi Fujimura

研究成果: Article

5 引用 (Scopus)

抄録

Background. The mechanism by which stimulated neutrophils (polymorphonuclear leukocytes [PMNs]) damage pulmonary vascular endothelium was investigated. Methods. The ability of unstimulated and mechanically stimulated PMNs to adhere to pulmonary endothelial cells and, thereby, alter pulmonary vascular permeability was tested. Each series was conducted on 6 rats. To stimulate PMNs, they were agitated gently in a glass vial for 10 seconds. Results. Perfusing lungs with the stimulated PMNs elicited a fivefold increase in permeability compared with lungs perfused with the unstimulated cells. This increase in permeability was blocked completely by preincubation of stimulated PMNs with CD18 monoclonal antibody. This increase in permeability was also blocked completely by superoxide dismutase (SOD) of the xanthine oxidase (XO) inhibitor allopurinol. Pulmonary vascular hemodynamics were unaffected by any treatment protocol. The accumulation of stimulated PMNs within the lungs was not inhibited by SOD but was partially blocked by allopurinol. Conclusions. These findings suggest that stimulated PMN-induced increases in pulmonary vascular filtration resulted from endothelial cell injury caused by superoxide anion possibly generated by XO, exclusively present in the endothelial cells.

元の言語English
ページ(範囲)402-407
ページ数6
ジャーナルAnnals of Thoracic Surgery
69
発行部数2
DOI
出版物ステータスPublished - 01-01-2000

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Lung Injury
Superoxides
Neutrophils
Endothelial Cells
Lung
Permeability
Allopurinol
Xanthine Oxidase
Superoxide Dismutase
Blood Vessels
Vascular Endothelium
Capillary Permeability
Clinical Protocols
Glass
Hemodynamics
Monoclonal Antibodies
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

これを引用

Tanita, Tatsuo ; Song, Chun ; Kubo, Hiroshi ; Hoshikawa, Yasushi ; Ueda, Shinsaku ; Fujimura, Shigefumi. / Superoxide possibly produced in endothelial cells mediates the neutrophil-induced lung injury. :: Annals of Thoracic Surgery. 2000 ; 巻 69, 番号 2. pp. 402-407.
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Superoxide possibly produced in endothelial cells mediates the neutrophil-induced lung injury. / Tanita, Tatsuo; Song, Chun; Kubo, Hiroshi; Hoshikawa, Yasushi; Ueda, Shinsaku; Fujimura, Shigefumi.

:: Annals of Thoracic Surgery, 巻 69, 番号 2, 01.01.2000, p. 402-407.

研究成果: Article

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