Suppression of hematopoietic activity in tenascin-C-deficient mice

Masatsugu Ohta, Takao Sakai, Yumiko Saga, Shin Ichi Aizawa, Masaki Saito

研究成果: Article査読

83 被引用数 (Scopus)

抄録

Tenascin-C (TN-C), a member of the extracellular matrix (ECM) glycoprotein family, is expressed on the surface of stromal cells in the hematopoietic system or lymphoid organs. Recently, TN-C-deficient mutant mice produced by TN-C gene targeting through homologous recombination were shown to develop normally, although TNs have been reported to play important roles in organogenesis and carcinogenesis. In the present study, we found that colony-forming capacity of bone marrow (BM) cells was considerably lower in TN-C-deficient mice (a decrease of ~35% from control), although their mononuclear cell count and BM architecture showed no significant difference from those of normal mice. Furthermore, in long-term BM culture in vitro, hematopoietic cell production (a decrease of ~40% in Dexter's condition and of ~65% in Whitlock-Witte's condition from control), colony-forming capacity of the produced cells (a decrease of ~60% from control), and longevity of the cultures were markedly lower in the TN-C-deficient mice than in control mice, whereas hematopoiesis in the TN-C-deficient mutant mice was sustained. The addition of TN-C glycoprotein to long-term BM cultures of TN-C-deficient mice clearly induced the recovery of hematopoietic cell production and colony-forming capacity of hematopoietic progenitor cells. Thus, these results provide direct evidence that an ECM glycoprotein component, TN-C, plays a relevant role in hematopoiesis through interactions between stromal cells and hematopoietic progenitor cells.

本文言語English
ページ(範囲)4074-4083
ページ数10
ジャーナルBlood
91
11
出版ステータスPublished - 01-06-1998

All Science Journal Classification (ASJC) codes

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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