Synergistic functions of phorbol ester and calcium in serotonin release from human platelets

Junji Yamanishi, Yoshimi Takai, Kozo Kaibuchi, Kimihiko Sano, Monique Castagna, Yasutomi Nishizuka

研究成果: Article査読

223 被引用数 (Scopus)

抄録

In human platelets, thrombin activates Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C) and mobilizes Ca2+ concomitantly, whereas 12-O-tetradecanoylphorbol-13-acetate (TPA) may be intercalated into membranes and directly activates protein kinase C without mobilization of Ca2+ in sufficient quantities. A series of experiments with TPA and Ca2+-ionophore (A23187) indicates that activation of protein kinase C is a prerequisite requirement for release of serotonin, and that this enzyme activation and Ca2+ mobilization act synergistically to elicit a full cellular response. Both cyclic AMP and cyclic GMP inhibit activation of protein kinase C by prohibiting the signal-dependent breakdown of inositol phospholipid to produce diacylglycerol, but none of these cyclic nucleotides prevents the TPA-induced activation of this enzyme.

本文言語English
ページ(範囲)778-786
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
112
2
DOI
出版ステータスPublished - 29-04-1983
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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