TY - JOUR
T1 - Synergistic functions of phorbol ester and calcium in serotonin release from human platelets
AU - Yamanishi, Junji
AU - Takai, Yoshimi
AU - Kaibuchi, Kozo
AU - Sano, Kimihiko
AU - Castagna, Monique
AU - Nishizuka, Yasutomi
N1 - Funding Information:
*/ This investigation was supported in part by research grants from the Scientific Research Fund of the Ministry of Education, Science and Culture,
PY - 1983/4/29
Y1 - 1983/4/29
N2 - In human platelets, thrombin activates Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C) and mobilizes Ca2+ concomitantly, whereas 12-O-tetradecanoylphorbol-13-acetate (TPA) may be intercalated into membranes and directly activates protein kinase C without mobilization of Ca2+ in sufficient quantities. A series of experiments with TPA and Ca2+-ionophore (A23187) indicates that activation of protein kinase C is a prerequisite requirement for release of serotonin, and that this enzyme activation and Ca2+ mobilization act synergistically to elicit a full cellular response. Both cyclic AMP and cyclic GMP inhibit activation of protein kinase C by prohibiting the signal-dependent breakdown of inositol phospholipid to produce diacylglycerol, but none of these cyclic nucleotides prevents the TPA-induced activation of this enzyme.
AB - In human platelets, thrombin activates Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C) and mobilizes Ca2+ concomitantly, whereas 12-O-tetradecanoylphorbol-13-acetate (TPA) may be intercalated into membranes and directly activates protein kinase C without mobilization of Ca2+ in sufficient quantities. A series of experiments with TPA and Ca2+-ionophore (A23187) indicates that activation of protein kinase C is a prerequisite requirement for release of serotonin, and that this enzyme activation and Ca2+ mobilization act synergistically to elicit a full cellular response. Both cyclic AMP and cyclic GMP inhibit activation of protein kinase C by prohibiting the signal-dependent breakdown of inositol phospholipid to produce diacylglycerol, but none of these cyclic nucleotides prevents the TPA-induced activation of this enzyme.
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U2 - 10.1016/0006-291X(83)91529-2
DO - 10.1016/0006-291X(83)91529-2
M3 - Article
C2 - 6405748
AN - SCOPUS:0020541662
SN - 0006-291X
VL - 112
SP - 778
EP - 786
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -