Synthesis and binding analysis of unique AG2 pentasaccharide to human Siglec-2 using NMR techniques

Shinya Hanashima, Ken ichi Sato, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Yukishige Ito, Yoshiki Yamaguchi

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Siglec-2 is a mammalian sialic acid binding protein expressed on B-cell surfaces and is involved in the modulation of B-cell mediated immune response. We synthesized a unique starfish ganglioside, AG2 pentasaccharide Galfβ(1-3)Galpα(1-4)Neu5Acα(2-3)Galpβ(1-4)Glcp, and found that the synthetic pentasaccharide binds to human Siglec-2 by performing 1H NMR experiments. Saturation transfer difference NMR experiments indicated that the C7-C9 side-chain and the acetamide moiety of the central sialic acid residue were located in the binding face of human Siglec-2. We determined the binding epitope of AG2 pentasaccharide to human Siglec-2, as the Galpα(1-4)Neu5Acα(2-3)Galp unit.

本文言語English
ページ(範囲)3720-3725
ページ数6
ジャーナルBioorganic and Medicinal Chemistry
18
11
DOI
出版ステータスPublished - 01-06-2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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