Systematic and quantitative identification of cellular glycan regulation using correlation index-based responsible gene screening (cires)

研究成果: Chapter

抄録

Cells manage the expression of their genomic contents to cope with their needs. In multicellular organisms, different cell types result from differential usage of the genome. In addition, cell surface glycans are often cell type specific and the mechanisms regulating glycan expression are controlled by gene expression, just like other cellular phenotypes. Here, a recently developed method for assessing the glycan-related genes that regulate cell surface glycans will be described. This method, called correlation index-based responsible enzyme gene screening (CIRES), uses statistical analysis of quantitative transcriptome data and glycan expression. Not only is this method useful for identifying the genes that positively regulate glycan expression, but CIRES can also systematically identify genes that negatively regulate glycan expression. CIRES involves three steps: (1) obtaining a glycan expression profile in several cell lines and gene expression profiles of glycan-related genes in the same set of cells, (2) correlating glycan and gene expression, and (3) evaluating the correlations in a gene perturbation study. Using this technique, one can determine the genes that quantitatively regulate the expression of cell surface glycans. This method complements conventional loss-of-function experiments, which can identify the exclusiveness of the gene for glycan expression.

本文言語English
ホスト出版物のタイトルGlycoscience
ホスト出版物のサブタイトルBiology and Medicine
出版社Springer Japan
ページ223-231
ページ数9
ISBN(電子版)9784431548416
ISBN(印刷版)9784431548409
DOI
出版ステータスPublished - 01-01-2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 医学(全般)

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