TAK-242 ameliorates contact dermatitis exacerbated by IL-36 receptor antagonist deficiency

Hidehiko Fukushima, Yohei Iwata, Soichiro Watanabe, Kenta Saito, Yoshihito Tanaka, Yurie Hasegawa, Masashi Akiyama, Kazumitsu Sugiura

研究成果: Article

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Loss-of-function mutations in IL36RN cause generalized pustular psoriasis (GPP), which is characterized by neutrophil-infiltrated lesions. Neutrophils are important during contact hypersensitivity in mice. However, it has never been determined whether interleukin-36 receptor antagonist (IL-36Ra) deficiency is an exacerbating factor in contact dermatitis. We examined whether a loss-of-function IL36RN mutation exacerbates contact dermatitis and evaluated the changes in contact dermatitis-related cytokines. Wild-type and Il36rn−/− mice were treated with 1-fluoro-2,4-dinitorobenzene (DNFB) and evaluated for ear thickness, histopathological features, numbers of infiltrated neutrophils, and numbers of CD4 + and CD8 + T cells. Furthermore, mRNA levels of contact dermatitis-related cytokines were measured by real-time polymerase chain reaction, and effects of TAK-242, a toll-like receptor 4 (TLR4) inhibitor, on the contact hypersensitivity (CHS) response were evaluated. We found that the ear thickness, cytokine expression, and neutrophil infiltration significantly increased in Il36rn−/− mice compared with that in wild-type mice. TAK-242 alleviated CHS and prevented neutrophil infiltration, cytokine expression, and ear thickening in Il36rn−/− mice. These data indicate that Il36rn−/− mutations are an exacerbating factor for CHS and that TAK-242 can reduce the inflammatory responses that are associated with the CHS response.

元の言語English
記事番号734
ジャーナルScientific reports
10
発行部数1
DOI
出版物ステータスPublished - 01-12-2020

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All Science Journal Classification (ASJC) codes

  • General

これを引用

Fukushima, H., Iwata, Y., Watanabe, S., Saito, K., Tanaka, Y., Hasegawa, Y., Akiyama, M., & Sugiura, K. (2020). TAK-242 ameliorates contact dermatitis exacerbated by IL-36 receptor antagonist deficiency. Scientific reports, 10(1), [734]. https://doi.org/10.1038/s41598-020-57550-5