Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest

Yoshiki Akatsuka, Eisei Kondo, Hirohumi Taji, Yasuo Morishima, Makoto Yazaki, Yuichi Obata, Yoshihisa Kodera, Stanley R. Riddell, Toshitada Takahashi

研究成果: ジャーナルへの寄稿学術論文査読

10 被引用数 (Scopus)

抄録

Background. T cells specific for minor histocompatibility antigens (mHAgs) play a major role in both graft-versus-host disease and the graft-versus-tumor effect after HLA-identical bone marrow transplantation (BMT). However, characterization of individual T-cell responses to mHAgs is difficult and has generally involved extensive screening of T-cell clones isolated from bulk T-cell cultures generated from BMT recipients. In this report, we describe a new approach that permits both direct visualization of CD8+ T-cell responses to mHAgs and cloning of T cells reacting with mHAgs presented by individual HLA alleles of interest. Methods and Results. Panels of Epstein-Barr virus-transformed B-cell lines (B-LCL) expressing retrovirally transduced HLA cDNA were used as stimulator cells in an enzyme-linked immunospot (ELISPOT) assay to identify CD8+ T cells reacting with mHAgs in cultures generated from postBMT recipient peripheral blood. T cells specific for mHAgs presented by selected HLA alleles could then be captured and cloned using an interferon-y secretion assay and magnetic bead selection. A majority of T-cell clones thus isolated exhibited cytolytic activity against the same HLA-transfected B-cell lines used for the ELISPOT assay. Conclusion. The ELISPOT assay was useful for identification of the HLA alleles presenting mHAgs recognized by individual T-cell lines. This approach for isolating mHAgs-specific CD8+ T-cell clones should assist in characterizing responses restricted by HLA alleles of interest, which are common in a certain ethnic group.

本文言語英語
ページ(範囲)1773-1780
ページ数8
ジャーナルTransplantation
74
12
DOI
出版ステータス出版済み - 27-12-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 移植

フィンガープリント

「Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル