TY - JOUR
T1 - Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum
AU - Seto, Yusuke
AU - Nakatani, Tomoya
AU - Masuyama, Norihisa
AU - Taya, Shinichiro
AU - Kumai, Minoru
AU - Minaki, Yasuko
AU - Hamaguchi, Akiko
AU - Inoue, Yukiko U.
AU - Inoue, Takayoshi
AU - Miyashita, Satoshi
AU - Fujiyama, Tomoyuki
AU - Yamada, Mayumi
AU - Chapman, Heather
AU - Campbell, Kenneth
AU - Magnuson, Mark A.
AU - Wright, Christopher V.
AU - Kawaguchi, Yoshiya
AU - Ikenaka, Kazuhiro
AU - Takebayashi, Hirohide
AU - Ishiwata, Shin'Ichi
AU - Ono, Yuichi
AU - Hoshino, Mikio
PY - 2014/2/18
Y1 - 2014/2/18
N2 - In the cerebellum, all GABAergic neurons are generated from the Ptf1a-expressing ventricular zone (Ptf1a domain). However, the machinery to produce different types of GABAergic neurons remains elusive. Here we show temporal regulation of distinct GABAergic neuron progenitors in the cerebellum. Within the Ptf1a domain at early stages, we find two subpopulations; dorsally and ventrally located progenitors that express Olig2 and Gsx1, respectively. Lineage tracing reveals the former are exclusively Purkinje cell progenitors (PCPs) and the latter Pax2-positive interneuron progenitors (PIPs). As development proceeds, PCPs gradually become PIPs starting from ventral to dorsal. In gain- and loss-of-function mutants for Gsx1 and Olig1/2, we observe abnormal transitioning from PCPs to PIPs at inappropriate developmental stages. Our findings suggest that the temporal identity transition of cerebellar GABAergic neuron progenitors from PCPs to PIPs is negatively regulated by Olig2 and positively by Gsx1, and contributes to understanding temporal control of neuronal progenitor identities.
AB - In the cerebellum, all GABAergic neurons are generated from the Ptf1a-expressing ventricular zone (Ptf1a domain). However, the machinery to produce different types of GABAergic neurons remains elusive. Here we show temporal regulation of distinct GABAergic neuron progenitors in the cerebellum. Within the Ptf1a domain at early stages, we find two subpopulations; dorsally and ventrally located progenitors that express Olig2 and Gsx1, respectively. Lineage tracing reveals the former are exclusively Purkinje cell progenitors (PCPs) and the latter Pax2-positive interneuron progenitors (PIPs). As development proceeds, PCPs gradually become PIPs starting from ventral to dorsal. In gain- and loss-of-function mutants for Gsx1 and Olig1/2, we observe abnormal transitioning from PCPs to PIPs at inappropriate developmental stages. Our findings suggest that the temporal identity transition of cerebellar GABAergic neuron progenitors from PCPs to PIPs is negatively regulated by Olig2 and positively by Gsx1, and contributes to understanding temporal control of neuronal progenitor identities.
UR - https://www.scopus.com/pages/publications/84894327725
UR - https://www.scopus.com/pages/publications/84894327725#tab=citedBy
U2 - 10.1038/ncomms4337
DO - 10.1038/ncomms4337
M3 - Article
C2 - 24535035
AN - SCOPUS:84894327725
SN - 2041-1723
VL - 5
JO - Nature communications
JF - Nature communications
M1 - 3337
ER -