Testosterone up-regulates aquaporin-4 expression in cultured astrocytes

Feng Gu, Ryuji Hata, Kazuko Toku, Lihua Yang, Yong Jie Ma, Nobuji Maeda, Masahiro Sakanaka, Junya Tanaka

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Aquaporin-4 (AQP4) is located on astrocyte endfeet that face blood vessels in the brain and in the pia. It is thought to play a crucial role in the development of brain edema. To confirm the notion that sex steroids and dexamethasone influence brain edema through AQP4 regulation, we investigated the effects of 17β-estradiol, testosterone, and dexamethasone on the expression of AQP4 in cultured astrocytes. Testosterone significantly up-regulated AQP4 at the level of both protein and mRNA. At a concentration of 100 nM, testosterone significantly increased AQP4 protein levels and ameliorated the osmotic fragility of astrocytes from hypoosmotic stress, suggesting that the increased levels of AQP4 facilitated the testosterone function. Moreover, this effect was attenuated by the protein kinase C activator 12-Otetradecanoylphorbol 13-acetate, which can rapidly decrease AQP4 mRNA expression, indicating that the response was specific. These results indicate that AQP4 can alter the osmotic fragility of astrocytes and that testosterone can influence brain edema through AQP4 regulation, whereas 17β-estradiol and dexamethasone cannot.

本文言語English
ページ(範囲)709-715
ページ数7
ジャーナルJournal of Neuroscience Research
72
6
DOI
出版ステータスPublished - 15-06-2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 細胞および分子神経科学

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