The 5′-triphosphates of 3′-azido-3′-deoxythymidine and 2′, 3′-dideoxynucleosides inhibit DNA polymerase γ by different mechanisms

Shunji Izuta, Mineo Saneyoshi, Takeshi Sakurai, Motoshi Suzuki, Kiyohide Kojima, Shonen Yoshida

研究成果: Article

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Inhibition mechanisms of 5′-triphosphates of 3′-azido-3′-deoxythymidine (AZT-TP) and 3′-deoxythymidine (ddTTP) on extensively purified DNA polymerase γ from bovine testes were examined by analysis of the products synthesized on singly primed M13 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase γ in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination. In contrast, both analogues were used by reverse transcriptase and caused chain termination.

元の言語English
ページ(範囲)776-783
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
179
発行部数2
DOI
出版物ステータスPublished - 16-09-1991

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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