The 5′-triphosphates of 3′-azido-3′-deoxythymidine and 2′, 3′-dideoxynucleosides inhibit DNA polymerase γ by different mechanisms

Shunji Izuta, Mineo Saneyoshi, Takeshi Sakurai, Motoshi Suzuki, Kiyohide Kojima, Shonen Yoshida

研究成果: Article

80 引用 (Scopus)

抄録

Inhibition mechanisms of 5′-triphosphates of 3′-azido-3′-deoxythymidine (AZT-TP) and 3′-deoxythymidine (ddTTP) on extensively purified DNA polymerase γ from bovine testes were examined by analysis of the products synthesized on singly primed M13 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase γ in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination. In contrast, both analogues were used by reverse transcriptase and caused chain termination.

元の言語English
ページ(範囲)776-783
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
179
発行部数2
DOI
出版物ステータスPublished - 16-09-1991
外部発表Yes

Fingerprint

Dideoxynucleosides
Zidovudine
DNA-Directed DNA Polymerase
splenotritin
DNA Primers
Single-Stranded DNA
RNA-Directed DNA Polymerase
DNA
Oligonucleotides
Thymidine
Testis
3'-azido-3'-deoxythymidine 5'-triphosphate
triphosphoric acid
2',3'-dideoxythymidine triphosphate

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

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abstract = "Inhibition mechanisms of 5′-triphosphates of 3′-azido-3′-deoxythymidine (AZT-TP) and 3′-deoxythymidine (ddTTP) on extensively purified DNA polymerase γ from bovine testes were examined by analysis of the products synthesized on singly primed M13 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase γ in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination. In contrast, both analogues were used by reverse transcriptase and caused chain termination.",
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The 5′-triphosphates of 3′-azido-3′-deoxythymidine and 2′, 3′-dideoxynucleosides inhibit DNA polymerase γ by different mechanisms. / Izuta, Shunji; Saneyoshi, Mineo; Sakurai, Takeshi; Suzuki, Motoshi; Kojima, Kiyohide; Yoshida, Shonen.

:: Biochemical and Biophysical Research Communications, 巻 179, 番号 2, 16.09.1991, p. 776-783.

研究成果: Article

TY - JOUR

T1 - The 5′-triphosphates of 3′-azido-3′-deoxythymidine and 2′, 3′-dideoxynucleosides inhibit DNA polymerase γ by different mechanisms

AU - Izuta, Shunji

AU - Saneyoshi, Mineo

AU - Sakurai, Takeshi

AU - Suzuki, Motoshi

AU - Kojima, Kiyohide

AU - Yoshida, Shonen

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