The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function

Atsushi Yokoyama, Ryuji Nomura, Masafumi Kurosumi, Atsushi Shimomura, Takanori Onouchi, Akiko Iizuka-Kogo, Ron Smits, Naohisa Oda, Riccardo Fodde, Mitsuyasu Itoh, Takao Senda

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc 1638T/1638T mice, which express a truncated Apc that lacks the C-terminal domain. Apc 1638T/1638T mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc 1638T/1638T mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc 1638T/1638T and Apc +/+ mice. Thyroid follicle size was significantly larger in Apc 1638T/1638T mice than in Apc +/+ mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injection was lower in Apc 1638T/1638T mice than in Apc +/+ mice. TSH also induced a greater reduction in thyroid follicle size in Apc 1638T/1638T mice than in Apc +/+ mice. Analyses using immunohistochemistry and electron microscopy indicated that follicular epithelial cells in Apc 1638T/1638T mice had an enlarged rough endoplasmic reticulum of irregular shape. These results suggest that the C-terminal domain of Apc is involved in thyroid morphology and function.

本文言語English
ページ(範囲)207-212
ページ数6
ジャーナルMedical Molecular Morphology
44
4
DOI
出版ステータスPublished - 12-2011

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

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