The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells

Joanne G.W. Nijhof, Kristin M. Braun, Adam Giangreco, Carina van Pelt, Hiroshi Kawamoto, Richard L. Boyd, Rein Willemze, Leon H.F. Mullenders, Fiona M. Watt, Frank R. de Gruijl, Willem van Ewijk

研究成果: Article査読

169 被引用数 (Scopus)

抄録

We describe a novel murine progenitor cell population localised to a previously uncharacterised region between sebaceous glands and the hair follicle bulge, defined by its reactivity to the thymic epithelial progenitor cell marker MTS24. MTS24 labels a membrane-bound antigen present during the early stages of hair follicle development and in adult mice. MTS24 co-localises with expression of α6-integrin and keratin 14, indicating that these cells include basal keratinocytes. This novel population does not express the bulge-specific stem cell markers CD34 or keratin 15, and is infrequently BrdU label retaining. MTS24-positive and -negative keratinocyte populations were isolated by flow cytometry and assessed for colony-forming efficiency. MTS24-positive keratinocytes exhibited a two-fold increase in colony formation and colony size compared to MTS24-negative basal keratinocytes. In addition, both the MTS24-positive and CD34-positive subpopulations were capable of producing secondary colonies after serial passage of individual cell clones. Finally, gene expression profiles of MTS24 and CD34 subpopulations were compared. These results showed that the overall gene expression profile of MTS24-positive cells resembles the pattern previously reported in bulge stem cells. Taken together, these data suggest that the cell-surface marker MTS24 identifies a new reservoir of hair follicle keratinocytes with a proliferative capacity and gene expression profile suggestive of progenitor or stem cells.

本文言語English
ページ(範囲)3027-3037
ページ数11
ジャーナルDevelopment
133
15
DOI
出版ステータスPublished - 08-2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 発生生物学

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