The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion

Noritoshi Kato, Yukio Yuzawa, Tomoki Kosugi, Akinori Hobo, Waichi Sato, Yuko Miwa, Kazuma Sakamoto, Seiichi Matsuo, Kenji Kadomatsu

研究成果: Article

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E-selectin and its ligands are essential for extravasation of leukocytes in inflammation. Here, we report that basigin (Bsg)/CD147 is a ligand for E-selectin that promotes renal inflammation in ischemia/ reperfusion. Compared with wild-type mice, Bsg-deficient (Bsg-/-) mice demonstrated striking suppression of neutrophil infiltration in the kidney after renal ischemia/reperfusion. Although E-selectin expression increased similarly between the two genotypes, Bsg-/- mice exhibited less renal damage, suggesting that Bsg on neutrophils contribute to renal injury in this model. Neutrophils expressed Bsg with N-linked polylactosamine chains and Bsg -/- neutrophils showed reduced binding to E-selectin. Bsg isolated from HL-60 cells bound to E-selectin, and tunicamycin treatment to abolish N-linked glycans from Bsg abrogated this binding. Furthermore, Bsg-/- neutrophils exhibited reduced E-selectin-dependent adherence to human umbilical vein endothelial cells in vitro. Injection of labeled neutrophils into mice showed that Bsg-/- neutrophils were less readily recruited to the kidney after renal ischemia/ reperfusion than Bsg+/+ neutrophils, regardless of the recipient's genotype. Taken together, these results indicate that Bsg is a physiologic ligand for E-selectin that plays a critical role in the renal damage induced by ischemia/reperfusion.

元の言語English
ページ(範囲)1565-1576
ページ数12
ジャーナルJournal of the American Society of Nephrology
20
発行部数7
DOI
出版物ステータスPublished - 07-2009

All Science Journal Classification (ASJC) codes

  • Nephrology

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