The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Akiko Morikawa, Naoki Koide, Tsuyoshi Sugiyama, Mya Mya Mu, Ferdaus Hassan, Shamima Islam, Hiroyasu Ito, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

本文言語English
ページ(範囲)211-218
ページ数8
ジャーナルFEMS Immunology and Medical Microbiology
41
3
DOI
出版ステータスPublished - 01-07-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 微生物学
  • 免疫学
  • 微生物学(医療)
  • 感染症

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