The inositol 5-phosphatase SHIP2 is an effector of RhoA and is involved in cell polarity and migration

Katsuhiro Kato, Tsubasa Yazawa, Kentaro Taki, Kazutaka Mori, Shujie Wang, Tomoki Nishioka, Tomonari Hamaguchi, Toshiki Itoh, Tadaomi Takenawa, Chikako Kataoka, Yoshiharu Matsuura, Mutsuki Amano, Toyoaki Murohara, Kozo Kaibuchi

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Cell migration is essential for various physiological and pathological processes. Polarization in motile cells requires the coordination of several key signaling molecules, including RhoA small GTPases and phosphoinositides. Although RhoA participates in a front-rear polarization in migrating cells, little is known about the functional interaction between RhoA and lipid turnover. We find here that src-homology 2-containing inositol-5-phosphatase 2 (SHIP2) interacts with RhoA in a GTP-dependent manner. The association between SHIP2 and RhoA is observed in spreading and migrating U251 glioma cells. The depletion of SHIP2 attenuates cell polarization and migration, which is rescued by wild-type SHIP2 but not by a mutant defective in RhoA binding. In addition, the depletion of SHIP2 impairs the proper localization of phosphatidylinositol 3,4,5-trisphosphate, which is not restored by a mutant defective in RhoA binding. These results suggest that RhoA associates with SHIP2 to regulate cell polarization and migration.

本文言語English
ページ(範囲)2593-2604
ページ数12
ジャーナルMolecular Biology of the Cell
23
13
DOI
出版ステータスPublished - 01-07-2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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