The occurrence of mouse-type oligosaccharides in mouse-human chimeric immunoglobulin G

Taei Matsui, Jiharu Hamako, Koh zoh Kameyama, Yoshikazu Kurosawa, Koiti Titani, Tsuguo Mizuochi

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The asparagine-linked sugar chains of mouse-human chimeric IgG which is composed of the variable regions derived from mouse and the constant regions derived from human and produced in mouse cells were quantitatively released as tritium-labelled oligosaccharides by hydrazinolysis followed by N-acetylation and NaB3H4-reduction. Paper electrophoresis in combination with sialidase digestion indicated that more than 70% of the oligosaccharides were neutral and the rest was sialylated oligosaccharides. Their structures were determined by sequential exoglycosidase digestions. The mouse-human chimeric IgG was shown to have same sugar chains as those of mouse IgG.

本文言語English
ページ(範囲)245-250
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
164
1
DOI
出版ステータスPublished - 16-10-1989

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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