The post-translational processing of ras p21 is critical for its stimulation of mitogen-activated protein kinase

Takahito Itoh, Kozo Kaibuchi, Tadayuki Masuda, Takeshi Yamamoto, Yoshiharu Matsuura, Akio Maeda, Kazuya Shimizu, Yoshimi Takai

研究成果: ジャーナルへの寄稿学術論文査読

72 被引用数 (Scopus)

抄録

The point-mutated active form of ras p21 is known to activate mitogen-activated protein (MAP) kinase/ extracellular signal-regulated kinase (ERK) in intact mammalian cells and Xenopus oocytes, although the direct target molecule of ras p21 remains to be identified. To elucidate the role of the post-translational processing of ras p21 for the MAP kinase activation, we established the cell-free system in which ras p21 activated MAP kinase. The guanosine 5′-(3-O-thio)triphosphate (GTPγS) bound form of post-translationally processed Ki-ras 4B p21 activated MAP kinase in the cytosol fraction of Xenopus oocytes, but the GTPγS bound form of post-translationally unprocessed Ki-ras 4B p21 or the GDP bound form of processed or unprocessed Ki-ras 4B p21 was far less effective. The GTPγS bound form of processed Ki-ros 4B p21 activated recombinant ERK2 in the presence of the cytosol fraction of Xenopus oocytes, but the unprocessed protein was far less effective. These results provide a complete biochemical assay for ras p21 to activate MAP kinase in a cell-free system and indicate that all the elements downstream of ras p21 necessary for the MAP kinase activation are cytosolic and that the post-translational processing of ras p21 is important for the MAP kinase activation.

本文言語英語
ページ(範囲)3025-3028
ページ数4
ジャーナルJournal of Biological Chemistry
268
5
出版ステータス出版済み - 15-02-1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「The post-translational processing of ras p21 is critical for its stimulation of mitogen-activated protein kinase」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル