The ret oncogene products are membrane-bound glycoproteins phosphorylated on tyrosine residues in vivo

Masahiko Taniguchi, Takashi Iwamoto, Michinari Hamaguchi, Mutsushi Matsuyama, Masahide Takahashi

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We identified ret oncogene products in NIH 3T3 cells transformed by the ret oncogene (NIH(ret)) and a cell line (Lym-ret) established from pre-B cell lymphoma which developed in Eμ-ret transgenic mice. Using the polyclonal antibody against the kinase domain of ret, two glycoproteins with apparent molecular weights of 100kd and 96kd were found in both cell lines, although the expression level of the 100kd protein was much higher than that of the 96kd protein. Cell fractionation experiments indicated that the 100kd protein was present predominantly in the membrane fraction while the 96kd protein was found in both membrane and cytosol fractions. Western blot analysis indicated that the 100kd ret protein was phosphorylated on tyrosine residues in vivo.

本文言語English
ページ(範囲)416-422
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
181
1
DOI
出版ステータスPublished - 27-11-1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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