The role of lymphocytes in the experimental progressive glomerulonephritis

Yohei Ikezumi, Katsue Kanno, Tamaki Karasawa, G. I. Dong Han, Yumi Ito, Hiroko Koike, Shinichi Toyabe, Makoto Uchiyama, Fujio Shimizu, Hiroshi Kawachi

研究成果: Article

23 引用 (Scopus)

抜粋

Background. Glomerular accumulation of leukocytes, including lymphocytes, is a common feature in most types of glomerulonephritis. However, the role of lymphocytes in progressive glomerulonephritis has not been elucidated. We examined the role of lymphocytes in the development of progressive mesangial proliferative glomerulonephritis induced by two injections of monoclonal antibody 1-22-3 in rats. Methods. To elucidate the role of lymphocytes, circulating lymphocytes were depleted using specific monoclonal antibodies to rat lymphocytes prior to the induction of progressive glomerulonephritis. The effects of lymphocyte depletion on proteinuria and glomerular alterations were assessed 7 and 56 days after the induction of progressive glomerulonephritis. Results. Significant glomerular accumulation of CD4+ T cells, CD8+ T cells, and ED3+-activated macrophage were observed after the induction of glomerulonephritis. Depletion studies showed that continuous treatment with anti-CD5, anti-CD4, or anti-CD8 treatment reduced proteinuria and ameliorated the glomerular lesions on day 56. Depletion of CD4+ T cells also reduced glomerular accumulation of CD8+ T cells and ED3+-activated macrophages, and reduced glomerular expression of mRNA for interferon-gamma (INF-γ) (63.0% in anti-CD5 and 62.3% reduction in anti-CD4). Transit lymphocyte depletion limited in early stage of progressive glomerulonephritis demonstrated that CD4+ T-cell depletion, but not anti-CD8 treatment prevented glomerular injuries 56 days after the induction of progressive glomerulonephritis. Conclusion. CD4+ T cells played a central role in the development of progressive glomerulonephritis, controlling recruitment and activation of CD8+ cytotoxic cells and/or macrophages.

元の言語English
ページ(範囲)1036-1048
ページ数13
ジャーナルKidney International
66
発行部数3
DOI
出版物ステータスPublished - 09-2004

All Science Journal Classification (ASJC) codes

  • Nephrology

フィンガープリント The role of lymphocytes in the experimental progressive glomerulonephritis' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Ikezumi, Y., Kanno, K., Karasawa, T., Dong Han, G. I., Ito, Y., Koike, H., Toyabe, S., Uchiyama, M., Shimizu, F., & Kawachi, H. (2004). The role of lymphocytes in the experimental progressive glomerulonephritis. Kidney International, 66(3), 1036-1048. https://doi.org/10.1111/j.1523-1755.2004.00852.x