TY - JOUR
T1 - Three distinct activities possibly involved in mRNA splicing are found in a nuclear fraction lacking U1 and U2 RNA
AU - Mayeda, Akila
AU - Tatei, Kazuaki
AU - Kitayama, Hitoshi
AU - Takemura, Kazuta
AU - Ohshima, Yasumi
N1 - Funding Information:
ACKNOWLEDGEMENT We are indebted to Dr. H. Tanaka of the National Chemical Laboratory for Industry and Prof. K. Murakami of our university for the support in the chemical synthesis of DNA. We would like to thank Dr. A. Ishiharaa of National Institute of Genetics for discussion, Dr. Joh-E Ikeda of National Institute of Agrobiological Resources for critical reading of the manuscript and Profs. Y. Watanabe and H. Hamaguchi of our university for encouragement. We also thank M. Itoh and N. Nagasu for discussion and help. This work was supported by grants from the Ministry of Education, Science and Culture of Japan.
PY - 1986/4/11
Y1 - 1986/4/11
N2 - A nuclear extract from HeLa cells was fractionated by DEAE-Sepharose chromatography, and the fractions were assayed for the binding activity for a small RNA transcript carrying a splice junction or branch point sequence. The binding activity for the RNA carrying a 5' splice junction was localized in the small nuclear ribonucleoprotein (snRNP) fraction together with binding activity for the RNA carrying a 3' splice junction or branch point sequence. However, stronger binding activities for the 3' splice junction RNA and for the branch point RNA were discovered in the flow-through fraction where no small nuclear RNA were detected. When the flow-through fraction was added to purified U1 ribonucleoprotein, the binding activity for the 5' splice junction RNA was markedly enhanced. We propose that the factors responsible for the three types of activities found in the flow-through fraction play a role in the splicing of mRNA precursor.
AB - A nuclear extract from HeLa cells was fractionated by DEAE-Sepharose chromatography, and the fractions were assayed for the binding activity for a small RNA transcript carrying a splice junction or branch point sequence. The binding activity for the RNA carrying a 5' splice junction was localized in the small nuclear ribonucleoprotein (snRNP) fraction together with binding activity for the RNA carrying a 3' splice junction or branch point sequence. However, stronger binding activities for the 3' splice junction RNA and for the branch point RNA were discovered in the flow-through fraction where no small nuclear RNA were detected. When the flow-through fraction was added to purified U1 ribonucleoprotein, the binding activity for the 5' splice junction RNA was markedly enhanced. We propose that the factors responsible for the three types of activities found in the flow-through fraction play a role in the splicing of mRNA precursor.
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U2 - 10.1093/nar/14.7.3045
DO - 10.1093/nar/14.7.3045
M3 - Article
C2 - 3008104
AN - SCOPUS:0022600434
SN - 0305-1048
VL - 14
SP - 3045
EP - 3057
JO - Nucleic acids research
JF - Nucleic acids research
IS - 7
ER -