Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration

Shujie Wang, Takashi Watanabe, Kenji Matsuzawa, Akira Katsumi, Mai Kakeno, Toshinori Matsui, Feng Ye, Kazuhide Sato, Kiyoko Murase, Ikuko Sugiyama, Kazushi Kimura, Akira Mizoguchi, Mark H. Ginsberg, John G. Collard, Kozo Kaibuchi

研究成果: Article査読

49 被引用数 (Scopus)

抄録

Migrating cells acquire front-rear polarity with a leading edge and a trailing tail for directional movement. The Rac exchange factor Tiam1 participates in polarized cell migration with the PAR complex of PAR3, PAR6, and atypical protein kinase C. However, it remains largely unknown how Tiam1 is regulated and contributes to the establishment of polarity in migrating cells. We show here that Tiam1 interacts directly with talin, which binds and activates integrins to mediate their signaling. Tiam1 accumulated at adhesions in a manner dependent on talin and the PAR complex. The interactions of talin with Tiam1 and the PAR complex were required for adhesion-induced Rac1 activation, cell spreading, and migration toward integrin substrates. Furthermore, Tiam1 acted with talin to regulate adhesion turnover. Thus, we propose that Tiam1, with the PAR complex, binds to integrins through talin and, together with the PAR complex, thereby regulates Rac1 activity and adhesion turnover for polarized migration.

本文言語English
ページ(範囲)331-345
ページ数15
ジャーナルJournal of Cell Biology
199
2
DOI
出版ステータスPublished - 15-10-2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • Cell Biology

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