Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Kazuki Nakajima, Yasuo Oda, Mitsuhiro Kinoshita, Takashi Masuko, Kazuaki Kakehi

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

本文言語English
ページ(範囲)972-976
ページ数5
ジャーナルAnalyst
127
7
DOI
出版ステータスPublished - 01-01-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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