Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Kazuki Nakajima, Yasuo Oda, Mitsuhiro Kinoshita, Takashi Masuko, Kazuaki Kakehi

    研究成果: ジャーナルへの寄稿学術論文査読

    6 被引用数 (Scopus)

    抄録

    We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

    本文言語英語
    ページ(範囲)972-976
    ページ数5
    ジャーナルAnalyst
    127
    7
    DOI
    出版ステータス出版済み - 2002

    All Science Journal Classification (ASJC) codes

    • 分析化学
    • 生化学
    • 環境化学
    • 分光学
    • 電気化学

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