Tissue and subcellular distributions of an inhibitory GDP GTP exchange protein (GDI) for smg p25A by use of its antibody

Hidetaro Nonaka, Kozo Kaibuchi, Kazuya Shimizu, Juro Yamamoto, Yoshimi Takai

研究成果: Article査読

10 被引用数 (Scopus)

抄録

We have recently purified from bovine brain cytosol to near homogeneity a GDP GTP exchange protein for smg p25A, named smg p25A GDI, that inhibits the dissociation of GDP from and the subsequent binding of GTP to smg p25A. In the present study, we made an antiserum against smg p25A GDI and studied its tissue distribution in rat and its subcellular distribution in rat cerebrum by use of this antiserum. smg p25A GDI was found in secretory cells with both regulated and constitutive secretion types. Since smg p25A was previously found in only secretory cells with a regulated secretion type, this result suggests that small GTP-binding proteins different from smg p25A but recognized by smg p25A GDI are present in secretory cells with a constitutive secretion type, and that smg p25A GDI is involved in both regulated and constitutive secretory processes. In subcellular fractionation analysis of rat cerebrum, smg p25A GDI was mostly found in the cytosol fraction of neuron body and synaptosome. In synaptosome, it was mainly found in the synaptic cytosol.

本文言語English
ページ(範囲)556-563
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
174
2
DOI
出版ステータスPublished - 31-01-1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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