TY - JOUR
T1 - Tissue and subcellular distributions of an inhibitory GDP/GTP exchange protein (GDI) for the RHO proteins by use of its specific antibody
AU - Shimizu, Kazuya
AU - Kaibuchi, Kozo
AU - Nonaka, Hidetaro
AU - Yamamoto, Juro
AU - Takai, Yoshimi
N1 - Funding Information:
I This investigation was supported by grants-in-aid for Scientific Research and Cancer Research from the Ministry of Education, Science, and Culture, Japan (1990), a grant-in-aid for Abnormalities in Hormone Receptor Mechanisms from the Ministry of Health and Welfare, Japan (1990), and by grants from the Human Frontier Science Program (1990), the Yamanouchi Foundation for Research on Metabolic Disease (1990), and the Research Program on Cell Calcium Signal in the Cardiovascular System (1990).
PY - 1991/2/28
Y1 - 1991/2/28
N2 - We have recently purified from bovine brain cytosol to near homogeneity a GDP/GTP exchange protein for the rho proteins, named rho GDI, that inhibits the dissociation of GDP from and the subsequent binding of GTP to the rho proteins. In the present study, we made a monoclonal antibody against rho GDI and studied its tissue distribution in rat and its subcellular distribution in rat cerebrum by use of this antibody. rho GDI was found in most rat tissues as described for the rho proteins. In rat cerebrum, rho GDI was mostly found in the cytosol of neuron body and synaptosome. In synaptosome, it was mainly found in the synaptic cytosol.
AB - We have recently purified from bovine brain cytosol to near homogeneity a GDP/GTP exchange protein for the rho proteins, named rho GDI, that inhibits the dissociation of GDP from and the subsequent binding of GTP to the rho proteins. In the present study, we made a monoclonal antibody against rho GDI and studied its tissue distribution in rat and its subcellular distribution in rat cerebrum by use of this antibody. rho GDI was found in most rat tissues as described for the rho proteins. In rat cerebrum, rho GDI was mostly found in the cytosol of neuron body and synaptosome. In synaptosome, it was mainly found in the synaptic cytosol.
UR - http://www.scopus.com/inward/record.url?scp=0026021430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026021430&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)81220-3
DO - 10.1016/S0006-291X(05)81220-3
M3 - Article
C2 - 1900152
AN - SCOPUS:0026021430
SN - 0006-291X
VL - 175
SP - 199
EP - 206
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -