Tolerance of different proteins for amino acid diversity

Motoshi Suzuki, Fred C. Christians, Baek Kim, Adonis Skandalis, Margaret E. Black, Lawrence A. Loeb

研究成果: Article

26 引用 (Scopus)

抜粋

Random mutagenesis of genes followed by positive genetic selection in bacteria requires that the variant molecules confer biological activity, and is thus the most demanding approach for generating new functionally active molecules. Furthermore, one can learn much about the protein in question by comparing the population of selected molecules to the library from which they were selected. Described here is a mathematical method designed to guide such comparisons. We use as examples the results of randomization-selection studies of four different proteins. There exists, in general, a positive correlation between the number of amino acid substitutions in a critical region of a protein and the likelihood of inactivation of that protein; a correlation long suspected, but developed here in detail. At this time, we are comparing regions in different proteins and our conclusions must be limited. However, the method presented can serve as a guideline for anticipating the yield of new active mutants in genetic complementation assays based on the extent of randomization.

元の言語English
ページ(範囲)111-118
ページ数8
ジャーナルMolecular Diversity
2
発行部数1-2
DOI
出版物ステータスPublished - 01-01-1996
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Information Systems
  • Molecular Biology
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

これを引用

Suzuki, M., Christians, F. C., Kim, B., Skandalis, A., Black, M. E., & Loeb, L. A. (1996). Tolerance of different proteins for amino acid diversity. Molecular Diversity, 2(1-2), 111-118. https://doi.org/10.1007/BF01718708