Toxoid flocculation assay by laser light-scattering

Masaaki Iwaki, Yoshinobu Horiuchi, Takako Komiya, Tadashi Fukuda, Yoshichika Arakawa, Motohide Takahashi

研究成果: ジャーナルへの寄稿学術論文査読

5 被引用数 (Scopus)

抄録

Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression.

本文言語英語
ページ(範囲)138-146
ページ数9
ジャーナルJournal of immunological methods
318
1-2
DOI
出版ステータス出版済み - 10-01-2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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