Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species

Fumio Takizawa, Kyosuke Araki, Maki Ohtani, Hideaki Toda, Yasutaka Saito, Veronica Soto Lampe, Johannes Martinus Dijkstra, Mitsuru Ototake, Tadaaki Moritomo, Teruyuki Nakanishi, Uwe Fischer

研究成果: ジャーナルへの寄稿学術論文査読

12 被引用数 (Scopus)


Eomesodermin (Eomes), a T-box transcription factor, is a key molecule associated with function and differentiation of CD8+ T cells and NK cells. Previously, two teleost Eomes genes (Eomes-a and -b), which are located on different chromosomes, were identified and shown to be expressed in zebrafish lymphocytes. For the present study, we identified these genes in rainbow trout and ginbuna crucian carp. Deduced Eomes-a and -b amino acid sequences in both fish species contain a highly conserved T-box DNA binding domain. In RT-PCR, both Eomes transcripts were readily detectable in a variety of tissues in rainbow trout and ginbuna. The high expression of Eomes-a and -b in brain and ovary suggests involvement in neurogenesis and oogenesis, respectively, while their expression in lymphoid tissues presumably is associated with immune functions. Investigation of separated lymphocyte populations from pronephros indicated that both Eomes-a and -b transcripts were few or absent in IgM+ lymphocytes, while relatively abundant in IgM-/CD8α+ and IgM-/CD8α- populations. Moreover, we sorted trout CD8α+ lymphocytes from mucosal and non-mucosal lymphoid tissues and compared the expression profiles of Eomes-a and -b with those of other T cell-related transcription factor genes (GATA-3, T-bet and Runx3), a Th1 cytokine gene (IFN-γ) and a Th2 cytokine gene (IL-4/13A). Interestingly, the tissue distribution of Eomes-a/b, T-bet, and Runx3 versus IFN-γ transcripts did not reveal simple correlations, suggesting tissue-specific properties of CD8α+ lymphocytes and/or multiple modes that drive IFN-γ expressions.

ジャーナルFish and Shellfish Immunology
出版ステータス出版済み - 01-2014

All Science Journal Classification (ASJC) codes

  • 環境化学
  • 免疫学
  • 水圏科学
  • 免疫学および微生物学(その他)


「Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。