Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles

Aya Yoshimura, Yoshitaka Tamai, Takahiro Ochiya

研究成果: Chapter

抄録

Extracellular vesicles (EVs) are mediators of intercellular communication in diverse cellular functions. Visualizing EVs in vivo is important to elucidate the biogenesis of EVs, and various approaches have been developed for in vivo EV tracking. The ubiquitously expressed tetraspanin CD63 is liberally incorporated into EVs. Thus, fluorescently tagged CD63 has been used in many studies to label EVs. In the present study, we presented isolation and transfer assays for EVs from two transgenic rats expressing CD63-GFP in their body fluids or brains.

本文言語English
ホスト出版物のタイトルExtracellular vesicles
編集者Sheila Spada, Lorenzo Galluzzi
出版社Academic Press Inc.
ページ231-242
ページ数12
ISBN(印刷版)9780128206621
DOI
出版ステータスPublished - 01-2020

出版物シリーズ

名前Methods in Enzymology
645
ISSN(印刷版)0076-6879
ISSN(電子版)1557-7988

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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