Trophic effect of β-amyloid precursor protein on cerebral cortical neurons in culture

Wataru Araki, Nobuya Kitaguchi, Yasuo Tokushima, Kazuhiro Ishii, Hisashi Aratake, Shun Shimohama, Shigenobu Nakamura, Jun Kimura

研究成果: Article査読

184 被引用数 (Scopus)

抄録

We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.

本文言語English
ページ(範囲)265-271
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
181
1
DOI
出版ステータスPublished - 27-11-1991

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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