TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation

Yuji Toiyama, Akira Mizoguchi, Kazushi Kimura, Junichirou Hiro, Yasuhiro Inoue, Tomonari Tutumi, Chikao Miki, Masato Kusunoki

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Aim: To investigate the expression patterns of TTYH2 in the human colon cancer and colon cancer cell lines and to evaluate the inhibitory effect of small interfering RNA (siRNA) on the expression of TTYH2 in colon cancer cell lines. Methods: We investigated the expression patterns of TTYH2 in colon cancer, adjacent non-tumorous colon mucosa, and cancer cell lines (DLD-1, caco-2, and Lovo) by RT-PCR. Furthermore, a siRNA plasmid expression vector against TTYH2 was constructed and transfected into DLD-1 and Caco-2 with Lipofectamine™ 2000. The down regulation of TTYH2 expression was detected by RT-PCR and the role of siRNA in inducing cell proliferation and cell aggregation was evaluated by MTT and aggregation assay. Results: TTYH2 gene expression in colon cancer tissue was significantly up-regulated compared with normal colonic mucosa (1.23 ± 0.404 vs 0.655 ± 0.373, P = 0.0103). Colon cancer derived cell lines including DLD-1, Caco-2, and Lovo also expressed high levels of TTYH2. In contrast, transfection with siRNA-TrYH2 significantly inhibited both proliferation and scattering of these cancer cell lines. Conclusion: The present work demonstrates, for the first time, that the TTYH2 gene expression is significantly up-regulated in colon cancer. The TTYH2 gene may play an important role in regulating both proliferating and metastatic potentials of colorectal cancer.

本文言語English
ページ(範囲)2717-2721
ページ数5
ジャーナルWorld Journal of Gastroenterology
13
19
DOI
出版ステータスPublished - 21-05-2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • 消化器病学

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