Tumor necrosis factor receptor-associated protein 1 regulates cell adhesion and synaptic morphology via modulation of N-cadherin expression

Kyoko Kubota, Kiyoshi Inoue, Ryota Hashimoto, Natsuko Kumamoto, Asako Kosuga, Masahiko Tatsumi, Kunitoshi Kamijima, Hiroshi Kunugi, Nakao Iwata, Norio Ozaki, Masatoshi Takeda, Masaya Tohyama

研究成果: Article査読

36 被引用数 (Scopus)

抄録

An increase in serum tumor necrosis factor-α (TNF-α) levels is closely related to the pathogenesis of major depression. However, the underlying molecular mechanism between this increase and impairment of brain function remains elusive. To better understand TNF-α/TNF receptor 1 signaling in the brain, we analyzed the brain distribution and function of tumor necrosis factor receptor-associated protein 1 (TRAP1). Here we show that TRAP1 is broadly expressed in neurons in the mouse brain, including regions that are implicated in the pathogenesis of major depression. We demonstrate that small interfering RNA-mediated knockdown of TRAP1 in a neuronal cell line decreases tyrosine phosphorylation of STAT3, followed by a reduction of the transcription factor E2F1, resulting in a down-regulation of N-cadherin, and affects the adhesive properties of the cells. In addition, in cultured hippocampal neurons, reduced expression of N-cadherin by TRAP1 knockdown influences the morphology of dendritic spines. We also report a significant association between several single nucleotide polymorphisms in the TRAP1 gene and major depression. Our findings indicate that TRAP1 mediates TNF-α/TNF receptor 1 signaling to modulate N-cadherin expression and to regulate cell adhesion and synaptic morphology, which may contribute to the pathogenesis of major depression.

本文言語English
ページ(範囲)496-508
ページ数13
ジャーナルJournal of neurochemistry
110
2
DOI
出版ステータスPublished - 07-2009

All Science Journal Classification (ASJC) codes

  • 生化学
  • 細胞および分子神経科学

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