Two-dimensional distribution of G(i2α) in the plasma membrane: A critical evaluation by immunocytochemistry

Ryuji Nomura, Chisato Inuo, Yukiko Takahashi, Tomiko Asano, Toyoshi Fujimoto

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.

本文言語English
ページ(範囲)139-144
ページ数6
ジャーナルFEBS Letters
415
2
DOI
出版ステータスPublished - 29-09-1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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