TY - JOUR
T1 - Two-dimensional distribution of G(i2α) in the plasma membrane
T2 - A critical evaluation by immunocytochemistry
AU - Nomura, Ryuji
AU - Inuo, Chisato
AU - Takahashi, Yukiko
AU - Asano, Tomiko
AU - Fujimoto, Toyoshi
N1 - Funding Information:
We are grateful to Mrs. Fujie Miyata for her secretarial assistance and to the Japanese Cancer Research Resources Bank for providing A431 cells. This work was supported by Grants-in-Aid for Scientific Research (B) (No. 08457001) of the Ministry of Education, Science, Sports, and Culture of the Japanese Government, and research grants from Ciba-Geigy Foundation (Japan) for the Promotion of Science and from The Naito Foundation (to T.F.), and by the Sasakawa Scientific Research Grant from the Japan Science Society (to R.N).
PY - 1997/9/29
Y1 - 1997/9/29
N2 - Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.
AB - Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.
UR - https://www.scopus.com/pages/publications/0031590153
UR - https://www.scopus.com/pages/publications/0031590153#tab=citedBy
U2 - 10.1016/S0014-5793(97)01114-9
DO - 10.1016/S0014-5793(97)01114-9
M3 - Article
C2 - 9350984
AN - SCOPUS:0031590153
SN - 0014-5793
VL - 415
SP - 139
EP - 144
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -