Two functionally different domains of rabphilin-3A, rab3A p25/smg p25A- binding and phospholipid- and Ca2+-binding domains

T. Yamaguchi, H. Shirataki, S. Kishida, M. Miyazaki, J. Nishikawa, K. Wada, S. I. Numata, K. Kaibuchi, Y. Takai

研究成果: Article査読

130 被引用数 (Scopus)

抄録

Rabphilin-3A is a putative target molecule for rab3A p25/smg p25A, which is a member of a ras p21-related small GTP-binding protein and implicated in neurotransmitter release from the synapse. Rabphilin-3A has two copies of an internal repeat that are homologous to the C2 domains of protein kinase C, synaptotagmin, and phospholipase A2, which are known to bind to phospholipid in a Ca2+-dependent manner. In the current study, we have investigated the functional domains of rabphilin-3A by use of three recombinant proteins as follows: full rabphilin-3A (1-704 amino acids), an N-terminal fragment (1- 280 amino acids), and a C-terminal fragment containing the C2 domains (281- 704 amino acids). Both rabphilin-3A and the C-terminal fragment bound to phospholipid in the presence of Ca2+, but the N-terminal fragment did not bind to phospholipid. 45Ca2+ bound to rabphilin-3A and the C-terminal fragment only in the presence of phospholipid but did not bind to the N- terminal fragment. The GTPγS-bound form of rab3A p25 bound to both rabphilin-3A and the N-terminal fragment but did not bind to the C-terminal fragment. These results indicate that rabphilin-3A has at least two functionally different domains, the N-terminal rab3A p25-binding and C- terminal phospholipid- and Ca2+-binding domains.

本文言語English
ページ(範囲)27164-27170
ページ数7
ジャーナルJournal of Biological Chemistry
268
36
出版ステータスPublished - 1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Two functionally different domains of rabphilin-3A, rab3A p25/smg p25A- binding and phospholipid- and Ca2+-binding domains」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル