Two-step binding of transcription factors causes sequential chromatin structural changes at the activated IL-2 promoter

Satoru Ishihara, Ronald H. Schwartz

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Most gene promoters have multiple binding sequences for many transcription factors, but the contribution of each of these factors to chromatin remodeling is still unclear. Although we previously found a dynamic change in the arrangement of nucleosome arrays at the Il2 promoter during T cell activation, its timing preceded that of a decrease in nucleosome occupancy at the promoter. In this article, we show that the initial nucleosome rearrangement was temporally correlated with the binding of NFAT1 and AP-1 (Fos/Jun), whereas the second step occurred in parallel with the recruitment of other transcription factors and RNA polymerase II. Pharmacologic inhibitors for activation of NFAT1 or induction of Fos blocked the initial phase in the sequential changes. This step was not affected, however, by inhibition of c-Jun phosphorylation, which instead blocked the binding of the late transcription factors, the recruitment of CREB-binding protein, and the acetylation of histone H3 at lysine 27. Thus, the sequential recruitment of transcription factors appears to facilitate two separate steps in chromatin remodeling at the Il2 locus.

本文言語English
ページ(範囲)3292-3299
ページ数8
ジャーナルJournal of Immunology
187
6
DOI
出版ステータスPublished - 15-09-2011

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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