Type VI collagen bound to collagen fibrils by chondroitin/dermatan sulfate glycosaminoglgcan in mouse corneal stroma

Makoto Nakamura, Satoshi Kimura, Miya Kobayashi, Koji Hirano, Takeshi Hoshino, Shinobu Awaya

研究成果: Article査読

15 被引用数 (Scopus)

抄録

We investigated the ultrastructural localization of type VI collagen in mouse corneal stroma and its relationship to striated collagen fibrils and glycosaminoglycans, using chondroitinase ABC digestion and immunoelectron microscopy with colloidal gold particles. After chondroitinase ABC digestion, the arrangement of striated collagen fibrils was disrupted, and large spaces containing widely scattered fibrils appeared. The spaces were filled by filamentous networks that were stained by anti-type VI collagen IgG, which were apparently clumps of beaded filament. Interfibrillar type VI collagen beaded filaments and immunogold particles decreased. Our results indicate that type VI collagen is bound to the striated collagen fibrils by mediation of chrondroitin/dermatan sulfate glycosaminoglycan or proteoglycan. We believe that this interaction is essential to the orderly arrangement of the striated collagen fibrils, which results in corneal transparency.

本文言語English
ページ(範囲)71-76
ページ数6
ジャーナルJapanese Journal of Ophthalmology
41
2
DOI
出版ステータスPublished - 01-03-1997

All Science Journal Classification (ASJC) codes

  • 眼科学

フィンガープリント

「Type VI collagen bound to collagen fibrils by chondroitin/dermatan sulfate glycosaminoglgcan in mouse corneal stroma」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル