Types of potassium channels involved in coronary reactive hyperemia depend on duration of preceding ischemia in rat hearts

Masanori Shinoda, Yukio Toki, Kichiro Murase, Shinji Mokuno, Kenji Okumura, Takayuki Ito

研究成果: Article査読

14 被引用数 (Scopus)

抄録

This study was undertaken to clarify factors other than nitric oxide involved in reactive hyperemia after a short(30 sec) and a long(300 sec) coronary global no-flow ischemia in isolated rat hearts perfused at a constant pressure(90 mmHg) with special focuses on the contribution of various K channels including large and small conductance Ca-activated K(KCa) channels as well as ATP-sensitive K(KATP) channels. Reactive hyperemia was induced following 30 sec and 300 sec of no-flow ischemia of the heart. Coronary reactive hyperemia was observed even after the inhibition of nitric oxide synthase by N(ω)-nitro-L-arginine methyl ester(L-NAME). Selected K channel blockers, none of which affected the basal flow, were used to evaluate contribution of K channels to this L-NAME-resistant reactive hyperemia. After 30-sec ischemia, tetraethylammonium(TEA: a non-selective K channel blocker), glibenclamide(Gli: a KATP channel blocker) and α,β-methylene adenosine 5'-diphosphonate(AOPCP: an inhibitor of ecto 5'-nucleotidase) all suppressed both peak flow/basal flow(%PF) and repayment of flow debt(%RFD); After 300-sec ischemia, TEA and charybdotoxin(ChTX: a large conductance KCa channel blocker) decreased %PF and %RFD; AOPCP decreased both %RFD and duration, 4-aminopyridine(a voltage-dependent K channel blocker) decreased only duration. Neither apamin(a small conductance KCa channel blocker) nor indomethacin(a cyclooxygenase inhibitor) affected the both types of reactive hyperemia. These findings suggest that opening of KATP channel contributes to coronary vasodilation in reactive hyperemia after short 30-sec ischemia, and that opening of KCa, but not KATP, channel contributes to it after long 300-sec ischemia. These results also suggest that adenosine may partly be involved in both types of reactive hyperemia.

本文言語English
ページ(範囲)997-1007
ページ数11
ジャーナルLife Sciences
61
10
DOI
出版ステータスPublished - 01-08-1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)

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