Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

Yoshimasa Maeno, Hisashi Fujioka, Michael R. Hollingdale, Christian F. Ockenhouse, Shusuke Nakazawa, Masamichi Aikawa

研究成果: Article

21 引用 (Scopus)

抄録

CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.

元の言語English
記事番号71100
ページ(範囲)383-390
ページ数8
ジャーナルExperimental Parasitology
79
発行部数3
DOI
出版物ステータスPublished - 01-01-1994
外部発表Yes

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Amelanotic Melanoma
Hep G2 Cells
Hepatocytes
Hepatocellular Carcinoma
Liver
Microvilli
Endothelial Cells
Sporozoites
Malaria
Immunohistochemistry
Monoclonal Antibodies
Antibodies

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Immunology
  • Infectious Diseases

これを引用

Maeno, Yoshimasa ; Fujioka, Hisashi ; Hollingdale, Michael R. ; Ockenhouse, Christian F. ; Nakazawa, Shusuke ; Aikawa, Masamichi. / Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells. :: Experimental Parasitology. 1994 ; 巻 79, 番号 3. pp. 383-390.
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abstract = "CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.",
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Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells. / Maeno, Yoshimasa; Fujioka, Hisashi; Hollingdale, Michael R.; Ockenhouse, Christian F.; Nakazawa, Shusuke; Aikawa, Masamichi.

:: Experimental Parasitology, 巻 79, 番号 3, 71100, 01.01.1994, p. 383-390.

研究成果: Article

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T1 - Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

AU - Maeno, Yoshimasa

AU - Fujioka, Hisashi

AU - Hollingdale, Michael R.

AU - Ockenhouse, Christian F.

AU - Nakazawa, Shusuke

AU - Aikawa, Masamichi

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AB - CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.

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