Ultrastructural study of Rac1 and its effectors beneath the substratum-facing membrane.

Takashi Watanabe, Shujie Wang, Mai Kakeno, Jiro Usukura, Kozo Kaibuchi

研究成果: ジャーナルへの寄稿学術論文査読

5 被引用数 (Scopus)

抄録

The cytoskeletal architecture and adhesion apparatus are tightly controlled during embryogenesis, tissue development, and carcinogenesis. The Rho family GTPases play central roles in regulation of the cytoskeleton and adhesions. Rac1, one of the Rho family GTPases, appears to be activated at the plasma membrane and exert its functions through its effectors. However, where Rac1 and its effectors function at the molecular level remains to be determined. In this study, we examined the molecular organization on the cytoplasmic surface of the substratum-facing plasma membrane, focusing on Rac1 and its effectors, IQGAP1 and Sra-1, by electron microscopy. We employed deep-etch immunoreplica methods to observe the membrane cytoskeletal architecture while determining molecular locations. Beneath the plasma membrane, Rac1 and its effectors showed similar, but distinct, destinations. Rac1 localized on the membrane and associated with the membrane cytoskeleton. IQGAP1 predominantly localized beside actin filaments and occasionally near microtubules together with Rac1. On the other hand, Sra-1 localized at actin filaments, microtubules, and the plasma membrane. Sra-1 colabeled with Rac1 was mainly found at the membrane and actin filaments. These results suggest that IQGAP1 and Sra-1 colocalize with Rac1 at distinct places, including the plasma membrane and cytoskeletal architecture, for their specific functions.

本文言語英語
ページ(範囲)101-107
ページ数7
ジャーナルCell structure and function
33
1
DOI
出版ステータス出版済み - 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 分子生物学
  • 細胞生物学

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