Up-regulated cytokine-inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients

Y. Nakajima, I. Tsuge, Y. Kondo, R. Komatsubara, N. Hirata, M. Kakami, M. Kato, H. Kurahashi, A. Urisu, Y. Asano

研究成果: Article

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Background: Although changes in the fine balance of allergen-specific T cells are crucial in the pathogenesis of allergic diseases, their roles in the allergic reaction to hen's eggs (HE) have not yet been fully analysed. Objective: Using microarray technology, allergen-stimulated T cells from HE-allergic children were analysed to identify genes that are specifically up-regulated in these cells. Methods: RNA from CD4+ CD14- cells, fractionated from allergen-stimulated peripheral mononuclear cells, was analysed using a whole -genome microarray and real-time RT-PCR. The protein expression of selected genes was ascertained by flow cytometry. Results: In microarray analyses of allergen-stimulated T cells, 43 genes were up-regulated in HE-allergic children but not in non-HE-allergic children. Among these, up-regulation of three genes, cytokine -inducible SH2-containing protein (CISH), nuclear factor of κ light polypeptide gene enhancer in B-cell inhibitor Z (NFKBIZ) and B-cell CLL/lymphoma 2 (BCL2), was confirmed by real-time quantitative RT-PCR. CISH, but not NFKBIZ or BCL2, showed a significantly higher ratio of antigen-stimulated cell transcription over unstimulated cells in HE-allergic than in non-HE-allergic children (P<0.01). Flow-cytometric analysis revealed that the percentage of CD25+CISH+ cells in CD4+ cells from patients with HE allergy was significantly higher than that in controls (P<0.01). The expression level of CISH was significantly higher in IL-4+ Th2 cells than in IFN- γ+ Th1 cells. Conclusion: We noted that CISH expression in allergen-stimulated CD4+ T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children.

元の言語English
ページ(範囲)1499-1506
ページ数8
ジャーナルClinical and Experimental Allergy
38
発行部数9
DOI
出版物ステータスPublished - 09-2008

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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