Up-regulation of telomerase in primary cultured rat hepatocytes

Katsura Nozawa, Yasuhiro Kurumiya, Akiko Yamamoto, Yuka Isobe, Motoshi Suzuki, Shonen Yoshida

研究成果: Article

21 引用 (Scopus)

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Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells, we examined telomerase activity in primary cultured rat hepatocytes. During culture under serum-free conditions, rat hepatocytes rapidly lose the ability of organ-specific expression of serum albumin, apolipoprotein A-I, and hepatocyte nuclear factor 4, and the capacity for cytochrome P-450 induction by xenobiotics. The telomerase activity was found to be concomitantly increased about 2.5-fold at 48 h and 3-fold at 72 h. Northern blot and RT-PCR analyses with primary cultured hepatocytes revealed the associated accumulation of rat telomerase RNA subunits (TR), and the mRNAs for a telomerase reverse transcriptase (TERT) and a telomerase-associated protein (TEP1). The activity of hepatocyte telomerase, which was elevated during the primary culture, increased further when the cells were stimulated with hepatocyte growth factor. In this case, however, the levels of TR, TERT, and TEP1 mRNA did not show any detectable changes.

元の言語English
ページ(範囲)361-367
ページ数7
ジャーナルJournal of Biochemistry
126
発行部数2
DOI
出版物ステータスPublished - 01-01-1999
外部発表Yes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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