USP40 deubiquitinates HINT1 and stabilizes p53 in podocyte damage

Shohei Takahashi, Daisuke Fukuhara, Toru Kimura, Toshiyuki Fukutomi, Eriko Tanaka, Naoaki Mikami, Ichiro Hada, Hiromu Takematsu, Yukino Nishibori, Yoshihiro Akimoto, Hiroshi Kiyonari, Takaya Abe, Otmar Huber, Kunimasa Yan

研究成果: ジャーナルへの寄稿学術論文査読

5 被引用数 (Scopus)

抄録

Podocyte damage is a major pathological lesion leading to focal segmental glomerulosclerosis (FSGS). Podocytes damaged by cellular stress undergo hypertrophy to compensate for podocytopenia. It is known that cyclin-dependent kinase inhibitors induced by p53 ensure podocytes hypertrophy; however, its precise mechanism remains to be further investigated. In this study, we found that ubiquitin specific protease 40 (USP40) is a novel regulator of p53. Although USP40 knockout mice established in the present study revealed no abnormal kidney phenotype, intermediate filament Nestin was upregulated in the glomeruli, and was bound to and colocalized with USP40. We also found that USP40 deubiquitinated histidine triad nucleotide-binding protein 1 (HINT1), an inducer of p53. Gene knockdown experiments of USP40 in cultured podocytes revealed the reduction of HINT1 and p53 protein expression. Finally, in glomerular podocytes of mouse FSGS, upregulation of HINT1 occurred in advance of the proteinuria, which was followed by upregulation of USP40, p53 and Nestin. In conclusion, USP40 bound to Nestin deubiquitinates HINT1, and in consequence upregulates p53. These results provide additional insight into the pathological mechanism of podocyte hypertrophy in FSGS.

本文言語英語
ページ(範囲)198-206
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
614
DOI
出版ステータス出版済み - 23-07-2022

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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