Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse

Takayuki Yamashita, Toshihide Hige, Tomoyuki Takahashi

研究成果: Article査読

128 被引用数 (Scopus)

抄録

Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5′-triphosphate (GTP) analog GTPγS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.

本文言語English
ページ(範囲)124-127
ページ数4
ジャーナルScience
307
5706
DOI
出版ステータスPublished - 07-01-2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

フィンガープリント

「Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル