@article{eab36338b221478a8b61ccdf2c7f6945,
title = "Visualization of complex DNA double-strand breaks in a tumor treated with carbon ion radiotherapy",
abstract = "Carbon ion radiotherapy shows great potential as a cure for X-ray-resistant tumors. Basic research suggests that the strong cell-killing effect induced by carbon ions is based on their ability to cause complex DNA double-strand breaks (DSBs). However, evidence supporting the formation of complex DSBs in actual patients is lacking. Here, we used advanced high-resolution microscopy with deconvolution to show that complex DSBs are formed in a human tumor clinically treated with carbon ion radiotherapy, but not in a tumor treated with X-ray radiotherapy. Furthermore, analysis using a physics model suggested that the complexity of radiotherapy-induced DSBs is related to linear energy transfer, which is much higher for carbon ion beams than for X-rays. Visualization of complex DSBs in clinical specimens will help us to understand the anti-tumor effects of carbon ion radiotherapy.",
author = "Takahiro Oike and Atsuko Niimi and Noriyuki Okonogi and Kazutoshi Murata and Akihiko Matsumura and Noda, {Shin Ei} and Daijiro Kobayashi and Mototaro Iwanaga and Keisuke Tsuchida and Tatsuaki Kanai and Tatsuya Ohno and Atsushi Shibata and Takashi Nakano",
note = "Funding Information: This work was supported by Grants-in-Aid from the Japan Society for the Promotion of Science for Exploratory Research KAKENHI [15K15447] and for Scientific Research (B) KAKENHI [24390288]. This work was also supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan for programs for Leading Graduate Schools, Cultivating Global Leaders in Heavy Ion Therapeutics and Engineering. Funding Information: Statistical analysis. The significance of the differences in the width of 53BP1 foci after carbon ion and X-ray radiotherapy was examined using the Mann–Whitney U test and SigmaPlot 13 software (Hulinks). Funding This work was supported by Grants-in-Aid from the Japan Society for the Promotion of Science for Exploratory Research KAKENHI [15K15447] and for Scientific Research (B) KAKENHI [24390288]. This work was also supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan for programs for Leading Graduate Schools, Cultivating Global Leaders in Heavy Ion Therapeutics and Engineering.",
year = "2016",
month = mar,
day = "1",
doi = "10.1038/srep22275",
language = "English",
volume = "6",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}