Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor

Toru Hirota, Tetsuro Morisaki, Yasuyuki Nishiyama, Tomotoshi Marumoto, Kenji Tada, Toshihiro Hara, Norio Masuko, Masaki Inagaki, Katsuyoshi Hatakeyama, Hideyuki Saya

研究成果: Article査読

167 被引用数 (Scopus)

抄録

The mitotic apparatus plays a pivotal role in dividing cells to ensure each daughter cell receives a full set of chromosomes and complement of cytoplasm during mitosis. A human homologue of the Drosophila warts tumor suppressor, h-warts/LATS1, is an evolutionarily conserved serine/threonine kinase and a dynamic component of the mitotic apparatus. We have identified an interaction of h-warts/LATS1 with zyxin, a regulator of actin filament assembly. Zyxin is a component of focal adhesion, however, during mitosis a fraction of cytoplasmic-dispersed zyxin becomes associated with h-warts/LATS1 on the mitotic apparatus. We found that zyxin is phosphorylated specifically during mitosis, most likely by Cdc2 kinase, and that the phosphorylation regulates association with h-warts/LATS1. Furthermore, microinjection of truncated h-warts/LATS1 protein, including the zyxin-binding portion, interfered with localization of zyxin to mitotic apparatus, and the duration of mitosis of these injected cells was significantly longer than that of control cells. These findings suggest that h-warts/LATS1 and zyxin play a crucial role in controlling mitosis progression by forming a regulatory complex on mitotic apparatus.

本文言語English
ページ(範囲)1073-1086
ページ数14
ジャーナルJournal of Cell Biology
149
5
DOI
出版ステータスPublished - 29-05-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • Cell Biology

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